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Optimizing hairy root production from explants of

Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE

《农业科学与工程前沿（英文）》 2020年 第7卷 第4期   页码 513-522 doi: 10.15302/J-FASE-2019272

摘要：

is a shrub that has been used in traditional herbal medicine. It has great pharmaceutical potential for treating diseases such as cancer and diabetes. As a prerequisite for propagation of this species on a large scale, hairy roots in were induced using and various factors affecting hairy root induction and growth evaluated. Seven factors were tested: (1) type of explant, (2) type of culture medium, (3) duration of pre-culture, (4) inoculum cell density, (5) duration of infection, (6) acetosyringone concentration in the culture medium, and (7) duration of incubation. The optimal protocol for hairy root induction and growth was: young shoots, pre-cultured in Y1 for 2 d, inoculated with broth with an OD of 0.6 for 20 min, and incubated for 3 d. Putative transgenic hairy roots were initially identified by morphology and then confirmed by polymerase chain reaction. Successful and optimal production of hairy roots is a critical prerequisite for industrial scale clonal propagation of . Being able to cultivate the plant on a large scale will provide rapid and ready supply of the plant materials that can be used in herbal medicine and in scientific and industrial exploitation.

关键词： hairy root induction     medicinal plant     MS growth medium     Phyllanthus hainanensis     Rhizobium rhizogenes    

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Effect of seismic wave propagation in massed medium on rate-dependent anisotropic damage growth in concrete

Alireza DANESHYAR, Hamid MOHAMMADNEZHAD, Mohsen GHAEMIAN

《结构与土木工程前沿（英文）》 2021年 第15卷 第2期   页码 346-363 doi: 10.1007/s11709-021-0694-z

摘要： Seismic modeling of massive structures requires special caution, as wave propagation effects significantly affect the responses. This becomes more crucial when the path-dependent behavior of the material is considered. The coexistence of these conditions renders numerical earthquake analysis of concrete dams challenging. Herein, a finite element model for a comprehensive nonlinear seismic simulation of concrete gravity dams, including realistic soil–structure interactions, is introduced. A semi-infinite medium is formulated based on the domain reduction method in conjunction with standard viscous boundaries. Accurate representation of radiation damping in a half-space medium and wave propagation effects in a massed foundation are verified using an analytical solution of vertically propagating shear waves in a viscoelastic half-space domain. A rigorous nonlinear finite element model requires a precise description of the material response. Hence, a microplane-based anisotropic damage–plastic model of concrete is formulated to reproduce irreversible deformations and tensorial degeneration of concrete in a coupled and rate-dependent manner. Finally, the Koyna concrete gravity dam is analyzed based on different assumptions of foundation, concrete response, and reservoir conditions. Comparison between responses obtained based on conventional assumptions with the results of the presented comprehensive model indicates the significance of considering radiation damping and employing a rigorous constitutive material model, which is pursued for the presented model.

关键词： soil–structure interaction     massed foundation     radiation damping     anisotropic damage    

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Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 216-219 doi: 10.1007/s11684-010-0037-2

摘要： To acquire data of Brucella cellular fatty acids (CFAs) and probe into the possibility of utilizing CFAs information in typing Brucella, 19 reference strains were subjected to CFAs study. After all strains were inoculated on Brucella Agar plates, the cells were harvested, saponificated, methylated and extracted to provide fatty acid methylesters for gas chromatography (GC) analysis. Based on the CFAs data matrix, a dendrogram of 19 reference strains was generated by SPSS11.5 software package. The results showed that 19 reference strains were divided into five clusters: cluster 1 included (bv. 1, 2, 3, 5) and ; cluster 2 included (bv. 3, 4, 5, 6) and (bv. 1, 2, 3); cluster 3 included (bv. 1, 2, 7, 9) and ; cluster 4 was (bv. 4); and cluster 5 was . Typing Brucella by GC analysis of CFAs is a good method to reflect drug resistance of Brucella, and the classification is beneficial for clinical therapy. It also provides a new result of typing and demonstrates that the traditional classification is not completely reasonable. CFAs analysis may identify (bv. 4) and .

关键词： Brucella     gas chromatography     cellular fatty acids     dendrogram     typing    

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Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

《医学前沿（英文）》 2009年 第3卷 第3期   页码 277-283 doi: 10.1007/s11684-009-0047-0

摘要： Using a standard cellular fusion technique and indirect enzyme-linked immunosorbent assay (ELISA), a hybridoma cell line strain secreting anti-HBs monoclonal antibody (mAb) (defined G6 mAb) was obtained. The cells grew and secreted mAb stably. Antibody titers in the culture supernatant and ascites were 2.048×10 and 4.096×10, respectively. By applying the anti-HBs G6 mAb and horseradish peroxidase (HRP)-labeled goat anti-HBs antibody, we developed a sandwich ELISA (defined G6m ELISA) for detecting both wild-type and immune escape mutant HBsAgs (IEM HBsAg). The assay was performed to detect 17 species of genome recombinant expression HBsAg, including two wild-type species and 15 IEM HBsAg species, which varied in the “a” determinant, in a group of patients infected with hepatitis B virus (HBV). The patients previously had a lower ELISA detection signal [(absorbance of patients/absorbance of normal people (P/N): 1.0―4.5)]. The results demonstrated that the sensitivity of this assay to wild-type HBsAg was no less than 0.125g/L; 12 of 15 IEM HBsAg species (P/N≥2.5) were positive for G6 mAb. Of the positive IEM HBsAg species, two had a low absorbance value at 450nm (), one had an intermediate value and nine had a high value, which was 7.55%(mean), 59.4% and 92.1%―109.4% of the wild-type value, respectively. The two species with low value and the three negative species mutated at the bases 120―124 in the first loop of the HBV “a” determinant. Using the G6 ELISA and two commercial ELISA kits (A and B), 177 patients were tested. The G6 ELISA had a significantly higher detection rate than either commercial ELISAs (19.21% 14.89% and 6.21%, respectively; <0.01, <0.05, respectively).

关键词： hepatitis B virus     gene variation     hepatitis B surface antigen     immune escape     enzyme-linked immunosorbent assay    

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Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 185-191 doi: 10.1007/s11684-010-0040-7

摘要： The factors affecting case detection of tuberculosis (TB)/human immunodeficiency virus (HIV) co-infection cases were explored in order to provide evidence-based formulation of a TB/HIV co-infection control strategy in China. Four counties with different modes of HIV transmission and varying cooperation mechanisms between TB and HIV institutions were selected. HIV test among TB patients and TB examination among people living with HIV/acquired immunodeficiency syndrome (AIDS) were conducted. Patients also completed questionnaires on potential factors affecting TB/HIV co-infection. The results showed that 19 (1.7%) were HIV-positive among 1089 cases of people living with active TB who received HIV testing. Conversely, there were 126 (10%) cases of active TB among 1255 cases of people living with HIV/AIDS. Among the newly discovered 145 cases of TB/HIV co-infection, AIDS institutions found 86.9% of these co-infections and 14.1% came from TB institutes. In different registration categories, there were 31 (21.4%) cases of smear positive TB, 104 (71.7%) cases of smear negative TB, and extrapulmonary cases accounted for 10 (6.9%). Gender, occupation, marital status, ethnic group, education, HIV transmission route, CD4 level, and type of TB susceptible symptoms had significant effects on whether HIV/AIDS patients were co-infected with TB disease (<0.01). Sex, age, ethnic group, occupation, and type of tuberculosis had no significant influence on whether TB patients had HIV infection. AIDS institutions could find more TB/HIV co-infection patients in comparison with TB institutions, particularly in patients with smear negative pulmonary tuberculosis and extrapulmonary tuberculosis. Therefore, a cooperation mechanism should be built up between TB and HIV control systems, and routine TB checks should be conducted for HIV/AIDS patients. In areas where sex or drug use is the major transmission mode of HIV infection, antiretroviral therapy should be expanded, treatment adherence should be improved and patients’ immunity level should be increased in order to decrease TB incidence. Cough and expectoration of more than two weeks, bloody sputum, and recurrent fevers could be revealing symptoms in TB screening of HIV/AIDS patients.

关键词： tuberculosis/acquired immunodeficiency syndrome     dual infection     prevention and control     case detection     impact factors    

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LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

《化学科学与工程前沿（英文）》 2012年 第6卷 第4期   页码 436-442 doi: 10.1007/s11705-012-1222-4

摘要： More and more herbal medicines are found to be the substrates of drug transporters. In this paper, chromatography/tandem mass spectrometry (LC/MS/MS) combined with radioisotope method was used for the quantification of catalpol, a traditional Chinese medicine, to study the affinity relationship between herbal medicines and transporters. Catalpol uptake experiment was carried out by using several transporters (OAT1, OCT2, OAT3, OATP1B1 and OATP2B1). And samples were precipitated with methanol and quantified with LC/MS/MS. The results show that catalpol has a good affinity with OCT2-transfected S2 cells. After studying drug-drug interaction between catalpol and C-tetraethylammonium (TEA), we found that catalpol is able to facilitate TEA transport mediated by OCT2, suggesting that catalpol could probably be a new promoter of OCT2.

关键词： catalpol     LC/MS/MS     OCT2    

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A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

《医学前沿（英文）》 2010年 第4卷 第2期   页码 204-207 doi: 10.1007/s11684-010-0043-4

摘要： Pooling field specimens could reduce the number of assay and thus increase the efficiency in detecting and screening pathogen infections by polymerase chain reaction (PCR)-based assay. We investigated a pooling strategy in diagnosis of in Two settings of specimens were prepared, divided into portions and detected by multiplex PCR. Specimens A was 0.4490 g positive lung tissue of 28 larval nodes from four snails mixed with 1.310€g negative lung tissue from six snails and divided into 32 portions. Specimens B was 0.5448 g positive lung tissue with 26 larval nodes from two snails mixed with 1.092 g negative lung tissue from seven snails and divided into 48 portions. Repeated sampling was performed and sample size-accumulated positive rate curves were drawn. According to the sample size-accumulated positive rate curves, the appropriate sample size of the two specimens were 18 and 15, respectively, which is 0.36–0.58 to the total sample size. These test characteristics and the relevant factors to the sample size would need to be determined in much larger studies and more appropriately in field populations. The result indicates that the number of larval node is not the most important, nor the only factor to the sample size. It also implies the feasibility to detect in by pooling strategies.

关键词： Angiostrongylus cantonensis     Pomacea canaliculata     multiplex polymerase chain reaction     diagnosis     pooling    

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大中功率节能调速传动的合理电压等级

马小亮

《中国工程科学》 2001年 第3卷 第11期   页码 80-84

摘要：

大中功率风机和泵采用变频调速可节约大量电能，大部分功率在0.2～2 MW范围中。我国现在200 kW以上的电机多是中压，现行中压电网多为10 kV，选用10 kV直接变频从技术和经济角度看都不太合理。由于变频器输入侧都有变压器，因此电机和变频器没有必要和电网一致。文章讨论不同功率段的合理电压等级以及在电机电压和电网电压不同情况下当变频器出现故障时如何实现旁路工作。

关键词： 大中功率     节能调速     合理电压等级     低压变频     中压变频     旁路    

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Uncoupled state space solution to layered poroelastic medium with anisotropic permeability and compressible

Zhiyong AI, Wenze ZENG, Yichong CHENG, Chao WU

《结构与土木工程前沿（英文）》 2011年 第5卷 第2期   页码 171-179 doi: 10.1007/s11709-011-0103-0

摘要： This paper presents an uncoupled state space solution to three-dimensional consolidation of layered poroelastic medium with anisotropic permeability and compressible pore fluid. Starting from the basic equations of poroelastic medium, and introducing intermediate variables, the state space equation usually comprising eight coupled state vectors is uncoupled into two sets of equations of six and two state vectors in the Laplace-Fourier transform domain. Combined with the continuity conditions between adjacent layers and boundary conditions, the uncoupled state space solution of a layered poroelastic medium is obtained by using the transfer matrix method. Numerical results show that the anisotropy of permeability and the compressibility of pore fluid have remarkable influence on the consolidation behavior of poroelastic medium.

关键词： uncoupled state space solution     layered poroelastic medium     three-dimensional consolidation     anisotropic permeability     compressible pore fluid    

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印度洋8.7级与8.5级地震的物理前兆

孙威,孙晓明

《中国工程科学》 2008年 第10卷 第2期   页码 14-25

摘要：

采用自主研发的地震应力仪、谐振仪 、倾斜仪和大地电位仪记录到印度洋8.7级大震的地震前兆——孕震物理模型和临震信号， 印度洋8.7级地震前后和8.5级地震前后的地壳受力情况有很大差异，表明 印度洋8.7级地震与8.5级地震的成因有着本质的区别，分析了海啸形成的原因，还发现了在 8.7级地震的孕育过程中有地下物质迁移迹象。

关键词： 印度洋8.7级与8.5级地震     临震信号     顺势脉冲     逆势脉冲    

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The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

《环境科学与工程前沿（英文）》 2022年 第16卷 第8期 doi: 10.1007/s11783-022-1529-4

摘要：

• The resistance of phage PhiX174 to nZVI was much stronger than that of MS2.

关键词： Nanoscale zero-valent iron (nZVI)     MS2     PhiΧ174     Resistance     Inactivation     Pathogenic microorganisms    

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Preparation of solar selective absorbing CuO coating for medium temperature application

HUANG Qunwu, WANG Yiping, LI Jinhua

《化学科学与工程前沿（英文）》 2007年 第1卷 第3期   页码 256-260 doi: 10.1007/s11705-007-0046-0

摘要： A new method of preparing CuO solar selective absorbing coating for medium temperature is presented. After pretreatment, brass was overlaid with CuO by chemical plating. The effects of reactant concentration, reaction temperature and reaction time on the absorptivity of CuO coating were investigated. The optimized condition of preparing CuO coating was obtained. The CuO coating was analyzed with X-ray photoelectron spectroscopy (XPS) and scanning electron microscopy (SEM). In order to prolong the period of use, the CuO coating was protected by TiO. The experiment shows that the TiO/CuO coating is more heat-resistant, acid-resistant, and wear resistant than CuO coating, without losing absorptivity markedly. The TiO coating can reduce emissivity and protect the CuO coating.

关键词： photoelectron spectroscopy     temperature     pretreatment     absorptivity     microscopy    

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Frontier of continuous structural health monitoring system for short & medium span bridges and condition

Ayaho MIYAMOTO, Risto KIVILUOMA, Akito YABE

《结构与土木工程前沿（英文）》 2019年 第13卷 第3期   页码 569-604 doi: 10.1007/s11709-018-0498-y

摘要： It is becoming an important social problem to make maintenance and rehabilitation of existing short and medium span(10-20 m) bridges because there are a huge amount of short and medium span bridges in service in the world. The kernel of such bridge management is to develop a method of safety(condition) assessment on items which include remaining life and load carrying capacity. Bridge health monitoring using information technology and sensors is capable of providing more accurate knowledge of bridge performance than traditional strategies. The aim of this paper is to introduce a state-of-the-art on not only a rational bridge health monitoring system incorporating with the information and communication technologies for lifetime management of existing short and medium span bridges but also a continuous data collecting system designed for bridge health monitoring of mainly short and medium span bridges. In this paper, although there are some useful monitoring methods for short and medium span bridges based on the qualitative or quantitative information, mainly two advanced structural health monitoring systems are described to review and analyse the potential of utilizing the long term health monitoring in safety assessment and management issues for short and medium span bridge. The first is a special designed mobile loading device(vehicle) for short and medium span road bridges to assess the structural safety(performance) and derive optimal strategies for maintenance using reliability based method. The second is a long term health monitoring method by using the public buses as part of a public transit system (called bus monitoring system) to be applied mainly to short and medium span bridges, along with safety indices, namely, “characteristic deflection” which is relatively free from the influence of dynamic disturbances due to such factors as the roughness of the road surface, and a structural anomaly parameter.

关键词： condition assessment     short & medium span bridge     structural health monitoring(SHM)     long-term data collection     system     maintenance     bridge performance     information technology     loading vehicle(public bus)     in-situ loading    

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Economic Growth Model Based on Six Basic Factors of Production—Xu Growth Rate Equation

Shou-bo Xu,Zong-yuan Huang

《工程管理前沿（英文）》 2014年 第1卷 第2期   页码 160-175 doi: 10.15302/J-FEM-2014024

摘要： This paper proposes an economic growth model based on the Six Basic Factors of Production and Xu Growth Rate Equation for the first time, which fills-in the deficits of the New Classical Economic Growth Model based on the Solow Model. The empirical analysis indicates that over the past three decades, the consumption of six basic factors of production had complicated relations with economic growth. In some years, the consumption rate of the basic factors of production was meager, but the economy witnessed relatively rocketing growth rate; in some years, the factors of production consumption rate was very high, but the economy growth rate slowed down. In general, economy grows at the expenses of huge consumption of four factors. There is an obvious characteristic of huge input and low efficiency. The average contribution rate of technological advance peaks in the middle, while it drops down at two ends. Noticeably, since 2004, the technology contribution rate generally shows a downward trend, reasons of which should be explored systematically in aspects like economic policy and industry structure with a view to boost further transformation of the macro-economy scientific growth model.

关键词： six basic factors of production     economic growth model     Xu Growth Rate Equation    

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大中型水利工程建设管理的实践和体会

张基尧

《中国工程科学》 2011年 第13卷 第8期   页码 22-25

摘要： 水利工程建设是基础设施建设的重要组成部分。加强水利工程建设管理是提高水利投资效益和社会、经济、生态效益的重要途经，是工程管理科学研究的重要内容。文章主要阐述了不同阶段大中型水利工程建设管理的责任主体、管理重点、有效做法和成功案例。

关键词： 大中型水利工程；项目管理；实践体会    

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标题 作者 时间 类型 操作

Optimizing hairy root production from explants of

Zhaogui YAN, Shengyu LIU, Junlian ZHANG, Guan HUANG, Lijun DUAN, Yaomei YE

期刊论文

Effect of seismic wave propagation in massed medium on rate-dependent anisotropic damage growth in concrete

Alireza DANESHYAR, Hamid MOHAMMADNEZHAD, Mohsen GHAEMIAN

期刊论文

Analysis of Brucella cellular fatty acids

Zhen-Xiang ZHAO MS, Hai JIANG MD, Bu-Yun CUI MS, Hong-Yan ZHAO, Dong-Ri PIAO MS, Lan-Yu LI, Su-Zhen HAOMS,

期刊论文

Preparation, identification, and clinical application of anti-HBs monoclonal antibody that binds both wild-type and immune escape mutant HBsAgs

Fanghe LI BM , Chunyan ZHANG MS , Jinghua LIU MS , Xiaoyan ZHANG MS , Bing YAN , Bo ZHANG MS , YongguoHUANG MS , Jingsong GONG BM , Yan CHEN BM ,

期刊论文

Study on factors affecting TB/HIV co-infection in four counties of China

Shi-Ming CHENG MD, Yu-Ji LAI MS, Er-Yong LIU MS, Lin ZHOU MD, Xue-Jing WANG MS, Qiu-Lan CHEN MS, Dong-MingLI MS, Ning WANG MD,

期刊论文

LC/MS/MS and radioisotope method combined for recognizing the affinity between catalpol and OCT2 transporter

Zhiyu ZHANG, Changxiao LIU, Duanyun SI, Rong LU, Xiulin YI

期刊论文

A pooling strategy of a PCR-based assay to detect Angiostrongylus cantonensis in snail intermediate host, Pomacea canaliculata

Fu-Rong WEI MS, Shan LV PhD, He-Xiang LIU, Ling HU, Yi ZHANG MS,

期刊论文

大中功率节能调速传动的合理电压等级

马小亮

期刊论文

Uncoupled state space solution to layered poroelastic medium with anisotropic permeability and compressible

Zhiyong AI, Wenze ZENG, Yichong CHENG, Chao WU

期刊论文

印度洋8.7级与8.5级地震的物理前兆

孙威,孙晓明

期刊论文

The inactivation of bacteriophages MS2 and PhiX174 by nanoscale zero-valent iron: Resistance difference

期刊论文

Preparation of solar selective absorbing CuO coating for medium temperature application

HUANG Qunwu, WANG Yiping, LI Jinhua

期刊论文

Frontier of continuous structural health monitoring system for short & medium span bridges and condition

Ayaho MIYAMOTO, Risto KIVILUOMA, Akito YABE

期刊论文

Economic Growth Model Based on Six Basic Factors of Production—Xu Growth Rate Equation

Shou-bo Xu,Zong-yuan Huang

期刊论文

大中型水利工程建设管理的实践和体会

张基尧

期刊论文